Will of Diogo de Sousa, by which he states that he had made a previous testament together with his late wife Madalena de Vasconcelos. Diogo outilines that all the instructions contained in that document should be carried out. He also stated that an entail was to be instituted, which would include 200,000 réis in public debt interests that would be purchased. The first administrator of the entail would be his nephew Francisco da Silva, son of his sister Antónia da Sousa. If he had no legitimate children, the entail would go to António de Sousa, nephew of the founder. If neither of them produced a legitimate heir, the entail would go to the next of kin, as long as they were descended from the Sousa. All administrators were obliged to use the Sousa name and were forbidden to marry Jews, Moors or Gentiles. The administrators of this entail and of the entail established by his father were allowed to be buried in the chapel in the sacristy of the S. Francisco do Porto monastery, except for the place where the testator and his wife were to be buried, which could never be touched again. The first administrator was obliged to give "tenças" from the rents of the entail to Isabel de Madureira and Helena do Paraíso, nuns of the convent of Santa Clara do Porto.

Followed by the approval deed (1625-05-20, fls. 267-268v).